Wednesday, December 17, 2014

Pollen competition... couple different ways to do it

There are a couple different ways to set up a pollen competition. One is to mix equal amounts of the competing pollen together and dip the stigma into it. Another common way is to cover half a virgin stigma with pollen of one type, and the other half with pollen of a second type. Here, we've done this second type of pollen competition with Columbia-GUS pollen and Landsberg pollen on a Columbia pistil, and stained for gus.

Monday, December 1, 2014

Tracking a small number of pollen tubes

Again, we are trying out different microscopes to track pollen tube progress. Here, a Columbia accession pistil has been pollinated with a small amount of Col pollen that contain the Lat52:GUS construct that turns pollen tubes blue. The pistil was fixed and stained after 24 hours. Images (20X resolution) were obtained as a z-stack, and collapsed into a single plane to give a fairly high resolution image of the whole pistil.